PIN4 Protein (AA 1-156) (His tag)

Produktnummer ABIN667897

Recombinant PIN4-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für PIN4 Protein (AA 1-156) (His tag) (ABIN667897)

Target: PIN4 (Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting 4 Protein (PIN4))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Reinheit: > 90 %

Produktdetails

Proteineigenschaft: AA 1-156

Aufreinigungstag / Konjugat: Dieses PIN4 Protein ist gelabelt mit His tag.

Sequenz: MGSSHHHHHH SSGLVPRGSH MPMAGLLKGL VRQLEQFRVQ QQASKMPPKG KSGSGKAGKG GAASGSDSAD KKAQGPKGGG NAVKVRHILC EKHGKIMEAM EKLKSGMRFN EVAAQYSEDK ARQGGDLGWM TRGSMVGPFQ EAAFALPVSG MDKPVFTDPP VKTKFGYHII MVEGRK

Produktmerkmale: PIN4, 1-156aa, Human, His tag, E.coli

Aufreinigung: > 90% by SDS-PAGE

Biological Activity Comment: Specific activity is > 700nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 1 mM DTT, 0.1 mM PMSF

Handhabung: Avoid repeated freezing and thawing cycles

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: PIN4 (Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting 4 Protein (PIN4))

Andere Bezeichnung: PIN4

Hintergrund: PIN4, also known as Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4, is peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and is essential for cell cycle regulation. There are two different isoforms which are PAR14 and PAR17. PIN4/PAR17 was imported into mitochondria in a time and membrane potential-dependent manner, where it reached the mitochondrial matrix. Moreover, Pin4 protein was shown to bind to double-stranded DNA under physiological salt conditions. Recombinant human PIN4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Molekulargewicht: 18.8 kDa (176aa) confirmed by MALDI-TOF