S100A5 Protein (AA 1-92) (His tag)

Produktnummer ABIN667887

Dieses Recombinant S100A5-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für S100A5 Protein (AA 1-92) (His tag) (ABIN667887)

Target: S100A5 (S100 Calcium Binding Protein A5 (S100A5))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 90 %

Produktdetails

Proteineigenschaft: AA 1-92

Aufreinigungstag / Konjugat: Dieses S100A5 Protein ist gelabelt mit His tag.

Sequenz: MGSSHHHHHH SSGLVPRGSH METPLEKALT TMVTTFHKYS GREGSKLTLS RKELKELIKK ELCLGEMKES SIDDLMKSLD KNSDQEIDFK EYSVFLTMLC MAYNDFFLED NK

Produktmerkmale: S100A5, 1-92aa, Human, His tag, E.coli

Aufreinigung: > 90% by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 30 % glycerol, 0.1M NaCl, 0.1 mM PMSF

Handhabung: Avoid repeated freezing and thawing cycles

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: S100A5 (S100 Calcium Binding Protein A5 (S100A5))

Andere Bezeichnung: S100A5

Hintergrund: S100 calcium binding protein A5, also known as S100A5, is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A5 has a Ca2+ affinity 20- to 100-fold higher than the other S100 proteins studied under identical conditions. This protein also binds Zn2+ and Cu2+, and Cu2+ strongly which impairs the binding of Ca2+. S100A5 is expressed in very restricted regions of the adult brain. Recombinant human S100A5 protien, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molekulargewicht: 12.9 kDa (112aa) confirmed by MALDI-TOF

NCBI Accession: NP_002953

Pathways: S100 Proteine