UBE2K Protein (AA 1-200) (His tag)

Produktnummer ABIN7279311

Recombinant UBE2K-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für UBE2K Protein (AA 1-200) (His tag) (ABIN7279311)

Target: UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 95 %

Produktdetails

Proteineigenschaft: AA 1-200

Aufreinigungstag / Konjugat: Dieses UBE2K Protein ist gelabelt mit His tag.

Sequenz: MANI AVQRIKREFK EVLKSEETSK NQIKVDLVDE NFTELRGEIA GPPDTPYEGG RYQLEIKIPE TYPFNPPKVR FITKIWHPNI SSVTGAICLD ILKDQWAAAM TLRTVLLSLQ ALLAAAEPDD PQDAVVANQY KQNPEMFKQT ARLWAHVYAG APVSSPEYTK KIENLCAMGF DRNAVIVALS SKSWDVETAT ELLLSN

Produktmerkmale: UBE2K, 1-200aa, Human, His tag, E.coli

Aufreinigung: > 95% by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 7.5) containing 1 mM DTT, 10 % glycerol, 50 mM NaCl

Handhabung: Avoid repeated freezing and thawing cycles

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))

Andere Bezeichnung: E2-25K/UBE2K

Hintergrund: Ubiquitin-conjugating enzyme E2 K isoform 1, also known as uBE2K, is a member of the ubiquitin-conjugating enzymes family. These enzymes participate in a variety of cellular processes, including selective protein degradation, DNA repair, cell cycle control, and sporulation. In this mechanism, the ATP-coupled activation and subsequent ligation of ubiquitin are catalyzed by separate enzymes functionally linked by ubiquitin carrier protein uBC1. Also, uBE2K is reportedly involved in Alzheimer's disease, Huntington's disease and antigen processing through its interaction with amyloid-beta, huntingtin, and MHC-heavy chain proteins. Recombinant human uBE2K protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molekulargewicht: 26.5 kDa (236aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

NCBI Accession: NP_005330