HAGH Protein (AA 1-260) (His tag)
Kurzübersicht für HAGH Protein (AA 1-260) (His tag) (ABIN667841)
Target
Alle HAGH Proteine anzeigenProtein-Typ
Biologische Aktivität
Spezies
Quelle
Applikation
Reinheit
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Proteineigenschaft
- AA 1-260
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Aufreinigungstag / Konjugat
- Dieses HAGH Protein ist gelabelt mit His tag.
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Sequenz
- MGSSHHHHHH SSGLVPRGSH MGSHMKVEVL PALTDNYMYL VIDDETKEAA IVDPVQPQKV VDAARKHGVK LTTVLTTHHH WDHAGGNEKL VKLESGLKVY GGDDRIGALT HKITHLSTLQ VGSLNVKCLA TPCHTSGHIC YFVSKPGGSE PPAVFTGDTL FVAGCGKFYE GTADEMCKAL LEVLGRLPPD TRVYCGHEYT INNLKFARHV EPGNAAIREK LAWAKEKYSI GEPTVPSTLA EEFTYNPFMR VREKTVQQHA GETDPVTTMR AVRREKDQFK MPRD
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Produktmerkmale
- HAGH, 1-260aa, Human, His tag, E.coli
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Aufreinigung
- > 95% by SDS-PAGE
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Biological Activity Comment
- Specific activity is > 500unit/mg, and is defined as the amount of enzyme that reduce 1.0 umole of 5,5 -Dithiobis(2-nitrobenzoic acid) (DTNB) with reduced glutathione per minute at pH 7.5 at 37C.
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Hydroxyacylglutathione Hydrolase (HAGH) (AA 1-260) protein (GST tag)
HAGH Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
Hydroxyacylglutathione Hydrolase (HAGH) (AA 1-260) protein (His tag)HAGH Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Hydroxyacylglutathione Hydrolase (HAGH) (AA 50-308), (partial) protein (GST tag)HAGH Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant 90 % ELISA
Hydroxyacylglutathione Hydrolase (HAGH) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)HAGH Spezies: Human Wirt: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 0.5 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.5) containing 10 % glycerol
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Handhabung
- Avoid repeated freezing and thawing cycles
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- HAGH (Hydroxyacylglutathione Hydrolase (HAGH))
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Andere Bezeichnung
- Glyoxalase II
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Hintergrund
- HAGH is a member of the glyoxalase family and a thiolesterase which hydrolyses S-lactoyl-glutathione to reduced glutathione and D-lactate. This protein is a detoxifying enzyme of glycolysis byproduct methylglyoxal and a target of p63 and p73 and serves as a pro-survival factor of the p53 family. It exists only as a monomer and binds two zinc ions per subunit. Recombinant human HAGH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
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Molekulargewicht
- 31.4 kDa (284aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_001035517
Target
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