P24 Gag (AA 155-321) protein (His tag)

Produktnummer ABIN667810

Recombinant P24 Gag-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für P24 Gag (AA 155-321) protein (His tag) (ABIN667810)

Target: P24 Gag

Protein-Typ: Recombinant

Spezies: Human Immunodeficiency Virus (HIV)

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 80 %

Produktdetails

Proteineigenschaft: AA 155-321

Aufreinigungstag / Konjugat: His tag

Sequenz: WVKVVEEKA FSPEVIPMFS ALSEGATPQD LNTMLNTVGG HQAAMQMLKE TINEEAAEWD RLHPVHAGPI APGQMREPRG SDIAGTTSTL QEQIGWMTHN PPIPVGEIYK RWIILGLNKI VRMYSPTSIL DIRQGPKEPF RDYVDRFYKT LRAEQASQEV KNWMTETL

Produktmerkmale: HIV1-p24, 155-321aa, HIV-1, His tag, E.coli

Aufreinigung: > 80% by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 100 mM NaCl, 0.1 mM PMSF, 10 % glycerol

Handhabung: Avoid repeated freezing and thawing cycles

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: P24 Gag

Andere Bezeichnung: Gag p24

Substanzklasse: Viral Protein

Hintergrund: The HIV1 p24 protein performs highly complex orchestrated tasks during the assembly, budding maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag p24, the major capsid protein of the HIV-1 virion, has been used in clinical trials as one of the components of the HIV-1 vaccine because of the high degree of sequence conservation between different isolates. Recombinant HIV1-p24 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Molekulargewicht: 21.2 kDa (188aa) confirmed by MALDI-TOF