PYM Protein (AA 1-204) (His tag)

Produktnummer ABIN7529154

Dieses Recombinant PYM-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für PYM Protein (AA 1-204) (His tag) (ABIN7529154)

Target: PYM (WIBG) (Partner of Y14 and mago (WIBG))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 85 %

Produktdetails

Proteineigenschaft: AA 1-204

Aufreinigungstag / Konjugat: Dieses PYM Protein ist gelabelt mit His tag.

Sequenz: MEAAGSPAAT ETGKYIASTQ RPDGTWRKQR RVKEGYVPQE EVPVYENKYV KFFKSKPELP PGLSPEATAP VTPSRPEGGE PGLSKTAKRN LKRKEKRRQQ QEKGEAEALS RTLDKVSLEE TAQLPSAPQG SRAAPTAASD QPDSAATTEK AKKIKNLKKK LRQVEELQQR IQAGEVSQPS KEQLEKLARR RALEEELEDL ELGL

Produktmerkmale: WIBG, 1-204aa, Human, His tag, E.coli

Aufreinigung: > 85% by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol 0.1M NaCl ,0.1 mM PMSF

Handhabung: Avoid repeated freezing and thawing cycles

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: PYM (WIBG) (Partner of Y14 and mago (WIBG))

Andere Bezeichnung: WIBG

Hintergrund: WIBG has been identified as an interacting partner of Mago-Y14. The Mago-Y14 heterodimer is a core component of the EJC (exon junction complex) that is deposited on mRNAs as a consequence of splicing and influences postsplicing mRNA metabolism. This protein is a cytoplasmic RNA-binding protein that is excluded from the nucleus by Crm1. It interacts directly with Mago-Y14 by means of its N-terminal domain. Recombinant human WIBG protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molekulargewicht: 23.7 kDa (212aa) confirmed by MALDI-TOF

NCBI Accession: NP_115721