UBA3 Protein (AA 1-463) (His tag)
Kurzübersicht für UBA3 Protein (AA 1-463) (His tag) (ABIN667686)
Target
Alle UBA3 Proteine anzeigenProtein-Typ
Spezies
Quelle
Applikation
Reinheit
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Proteineigenschaft
- AA 1-463
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Aufreinigungstag / Konjugat
- Dieses UBA3 Protein ist gelabelt mit His tag.
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Sequenz
- MGSSHHHHHH SSGLVPRGSH MGSHMADGEE PERKRRRIEE LLAEKMAVDG GCGDTGDWEG RWNHVKKFLE RSGPFTHPDF EPSTESLQFL LDTCKVLVIG AGGLGCELLK NLALSGFRQI HVIDMDTIDV SNLNRQFLFR PKDIGRPKAE VAAEFLNDRV PNCNVVPHFN KIQDFNDTFY RQFHIIVCGL DSIIARRWIN GMLISLLNYE DGVLDPSSIV PLIDGGTEGF KGNARVILPG MTACIECTLE LYPPQVNFPM CTIASMPRLP EHCIEYVRML QWPKEQPFGE GVPLDGDDPE HIQWIFQKSL ERASQYNIRG VTYRLTQGVV KRIIPAVAST NAVIAAVCAT EVFKIATSAY IPLNNYLVFN DVDGLYTYTF EAERKENCPA CSQLPQNIQF SPSAKLQEVL DYLTNSASLQ MKSPAITATL EGKNRTLYLQ SVTSIEERTR PNLSKTLKEL GLVDGQELAV ADVTTPQTVL FKLHFTS
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Produktmerkmale
- UBA3, 1-463aa, Human, His tag, E.coli
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Aufreinigung
- > 90% by SDS-PAGE
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Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (AA 1-463) protein (GST tag)
UBA3 Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AA, AP
Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (AA 1-463) protein (His-IF2DI Tag)UBA3 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)UBA3 Spezies: Human Wirt: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)UBA3 Spezies: Human Wirt: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 1 mM DTT
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Handhabung
- Avoid repeated freezing and thawing cycles
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- UBA3 (Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3))
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Andere Bezeichnung
- UBA3
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Hintergrund
- UBA3, also known as NEDD8-activating enzyme E1 catalytic subunit, is the catalytic subunit of the dimeric uBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-uBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of uBE2M. Recombinant human uBA3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
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Molekulargewicht
- 54.4 kDa (487aa)
Target
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