3-Hydroxy-3-Methylglutaryl-Coenzyme A Lyase (HMGCL) protein (HIS)

Name: 3-Hydroxy-3-Methylglutaryl-Coenzyme A Lyase (HMGCL)

Hergestellt in: Escherichia Coli (E. coli)

Herkunft (Gen): Human

Konjugat: HIS

Applikation: SDS-PAGE (SDS)

Produktnummer: ABIN667639

Produktbeschreibung

Produktmerkmale: HMGCL, 28-325aa , Human, His tag, E.coli

Format: Liquid

Beschreibung: HMGCL, also known as hydroxymethylglutaryl-CoA lyase, is a mitochondrial matrix protein that belongs to the HMG-CoA lyase family. It exists as a homodimer and participates in leucine catabolism and ketogenesis, the hepatic synthesis of ketone bodies that, during fasting, provide a major source of energy for heart, brain and kidney. More specifically, it catalyzes the final step of these processes, the cleavage of 3-hydroxy-3-methylglutaryl-CoA to acetoacetic acid and acetyl-CoA. Recombinant human HMGCL protein, fused to His-tag at N-terminus, was expressed in E.coli. Synonyms: Hydroxymethylglutaryl-CoA lyase, 3-hydroxy-3-methylglutarate-CoA lyase, HL, HMG-CoA lyase, Hydroxymethylglutaryl-CoA lyase mitochondrial. NCBI no.: NP_000182

Molekulargewicht: 34.2 kDa (323aa)

Anwendungen

Konzentration: 1.0 mg/ml (determined by Bradford assay)

Reinheit: > 85% by SDS - PAGE

Buffer: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.4M Urea

Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Bilder

3-Hydroxy-3-Methylglutaryl-Coenzyme A Lyase (HMGCL) protein (HIS)