UBE2D4 Protein (AA 1-147) (His tag)

Produktnummer ABIN667583

Dieses Recombinant UBE2D4-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für UBE2D4 Protein (AA 1-147) (His tag) (ABIN667583)

Target: UBE2D4 (Ubiquitin-Conjugating Enzyme E2D 4 (UBE2D4))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 95 %

Produktdetails

Proteineigenschaft: AA 1-147

Aufreinigungstag / Konjugat: Dieses UBE2D4 Protein ist gelabelt mit His tag.

Sequenz: MALKRIQKEL TDLQRDPPAQ CSAGPVGDDL FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TKIYHPNINS NGSICLDILR SQWSPALTVS KVLLSICSLL CDPNPDDPLV PEIAHTYKAD REKYNRLARE WTQKYAM

Produktmerkmale: UBE2D4, 1-147aa, Human, His tag, E.coli

Aufreinigung: > 95% by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 2 mM DTT, 10 % glycerol, 100 mM NaCl

Handhabung: Avoid repeated freezing and thawing cycles

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: UBE2D4 (Ubiquitin-Conjugating Enzyme E2D 4 (UBE2D4))

Andere Bezeichnung: UBE2D4

Hintergrund: UBE2D4 belongs to the ubiquitin-conjugating enzyme family. ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, perform the second step in the ubiquitination reaction that targets a protein for degradation via the proteasome. uBE2D4 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination. Recombinant human uBE2D4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molekulargewicht: 18.8 kDa (167aa) confirmed by MALDI-TOF

NCBI Accession: NP_057067