Sntn Protein (AA 1-147) (His tag)

Produktnummer ABIN667444

Recombinant Sntn-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für Sntn Protein (AA 1-147) (His tag) (ABIN667444)

Target: Sntn (Sentan, Cilia Apical Structure Protein (Sntn))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 90 %

Produktdetails

Proteineigenschaft: AA 1-147

Aufreinigungstag / Konjugat: Dieses Sntn Protein ist gelabelt mit His tag.

Sequenz: MGSSHHHHHH SSGLVPRGSH MGGCMHSTQD KSLHLEGDPN PSAAPTSTCA PRKMPKRISI SKQLASVKAL RKCSDLEKAI ATTALIFRNS SDSDGKLEKA IAKDLLQTQF RNFAEGQETK PKYREILSEL DEHTENKLDF EDFMILLLSI TVMSDLLQNI RNVKIMK

Produktmerkmale: SNTN, 1-147aa, Human, His tag, E.coli

Aufreinigung: > 90% by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 50 % glycerol, 0.15M NaCl, 1 mM DTT

Handhabung: Avoid repeated freezing and thawing cycles

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: Sntn (Sentan, Cilia Apical Structure Protein (Sntn))

Andere Bezeichnung: SNTN

Hintergrund: SNTN, also known as sentan, belongs to the S-100 family. SNTN is shown to localize exclusively to the bridging structure between the cell membrane and peripheral singlet microtubules, which specifically exists in the narrowed distal portion of cilia. Exogenously expressed sentan showed affinity for the membrane protrusions, and a protein-lipid binding assay revealed that sentan bound to phosphatidylserine. These findings suggest that sentan is the first molecular component of the ciliary tip to bridge the cell membrane and peripheral singlet microtubules, making the distal portion of the cilia narrow and stiff to allow for better airway clearance or ovum transport. Recombinant human SNTN protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Molekulargewicht: 18.6 kDa (167aa) confirmed by MALDI-TOF

NCBI Accession: NP_001074006