5,10-Methenyltetrahydrofolate Synthetase (5-Formyltetrahydrofolate Cyclo-Ligase) (MTHFS) protein (HIS)

Name: 5,10-Methenyltetrahydrofolate Synthetase (5-Formyltetrahydrofolate Cyclo-Ligase) (MTHFS)

Synonyme: FLJ30410, HsT19268, AI119695, 1110034I12Rik, 2310020H23Rik, MGC95139, MTHFS

Hergestellt in: Escherichia Coli (E. coli)

Herkunft (Gen): Human

Konjugat: HIS

Applikation: SDS-PAGE (SDS)

Produktnummer: ABIN667396

Produktbeschreibung

Produktmerkmale: MTHFS, 1-203aa, Human, His tag, E.coli

Format: Liquid

Beschreibung: MTHFS, also known as 5-formyltetrahydrofolate cyclo-ligase, is a cytosolic protein involved in the formate metabolic process. MTHFS, with a magnesium cofactor, catalyzes the ATP-dependent reaction that reduces 5-formyltetrahydrofolate (5-MTHF) to 5,10-methenyltetrahydrofolate(MTHF). MTHF is the substrate used by MTHFR (methylenetetrahydrofolate reductase) to generate 5-MTHF. MTHF is also a coenzyme used in thymidine biosynthesis by thymidylate synthase (FAD). Recombinant human MTHFS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: 5-formyltetrahydrofolate cyclo-ligase, Methenyl-THF synthetase. NCBI no.: NP_006432

Molekulargewicht: 25.4kDa (223aa) confirmed by MALDI-TOF

Anwendungen

Konzentration: 0.5 mg/ml (determined by Bradford assay)

Reinheit: > 95% by SDS - PAGE

Buffer: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 30% glycerol, 0.2M NaCl

Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Bilder

5,10-Methenyltetrahydrofolate Synthetase (5-Formyltetrahydrofolate Cyclo-Ligase) (MTHFS) protein (HIS)