EIF1B Protein (AA 1-113) (His tag)

Produktnummer ABIN667384

Recombinant EIF1B-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für EIF1B Protein (AA 1-113) (His tag) (ABIN667384)

Target: EIF1B (Eukaryotic Translation Initiation Factor 1B (EIF1B))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 95 %

Produktdetails

Proteineigenschaft: AA 1-113

Aufreinigungstag / Konjugat: Dieses EIF1B Protein ist gelabelt mit His tag.

Sequenz: MGSSHHHHHH SSGLVPRGSH MSTIQNLQSF DPFADATKGD DLLPAGTEDY IHIRIQQRNG RKTLTTVQGI ADDYDKKKLV KAFKKKFACN GTVIEHPEYG EVIQLQGDQR KNICQFLLEV GIVKEEQLKV HGF

Produktmerkmale: EIF1B, 1-113aa, Human, His tag, E.coli

Aufreinigung: > 95% by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.5) containing 10 % glycerol, 2 mM DTT, 0.1M NaCl

Handhabung: Avoid repeated freezing and thawing cycles

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: EIF1B (Eukaryotic Translation Initiation Factor 1B (EIF1B))

Andere Bezeichnung: EIF1B

Hintergrund: EIF1B is crucial for the scanning process in vitro. During the scanning process, EIF1B is a component of a complex involved in recognition of the initiator codon. Translation is also initiated by the role of EIF1B in regulating the activity of ribosomal subunits 43S, 48S and 40S. This protein enables 43S ribosomal complexes to discern between cognate and near-cognate initiation codons, sensing the nucleotide content of initiation codons. Recombinant human EIF1B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molekulargewicht: 15 kDa (133aa) confirmed by MALDI-TOF

NCBI Accession: NP_005866