BCKDHA Protein (AA 46-445) (His tag)

Produktnummer ABIN667373

Dieses Recombinant BCKDHA-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für BCKDHA Protein (AA 46-445) (His tag) (ABIN667373)

Target: BCKDHA (Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 80 %

Produktdetails

Proteineigenschaft: AA 46-445

Aufreinigungstag / Konjugat: Dieses BCKDHA Protein ist gelabelt mit His tag.

Sequenz: MSSLDDKPQF PGASAEFIDK LEFIQPNVIS GIPIYRVMDR QGQIINPSED PHLPKEKVLK LYKSMTLLNT MDRILYESQR QGRISFYMTN YGEEGTHVGS AAALDNTDLV FGQYREAGVL MYRDYPLELF MAQCYGNISD LGKGRQMPVH YGCKERHFVT ISSPLATQIP QAVGAAYAAK RANANRVVIC YFGEGAASEG DAHAGFNFAA TLECPIIFFC RNNGYAISTP TSEQYRGDGI AARGPGYGIM SIRVDGNDVF AVYNATKEAR RRAVAENQPF LIEAMTYRIG HHSTSDDSSA YRSVDEVNYW DKQDHPISRL RHYLLSQGWW DEEQEKAWRK QSRRKVMEAF EQAERKPKPN PNLLFSDVYQ EMPAQLRKQQ ESLARHLQTY GEHYPLDHFD K

Produktmerkmale: BCKDHA, 46-445aa, Human, His tag, E.coli

Aufreinigung: > 80% by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 5 mM DTT, 30 % glycerol, 0.2M NaCl

Handhabung: Avoid repeated freezing and thawing cycles

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: BCKDHA (Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA))

Andere Bezeichnung: BCKDHA

Hintergrund: BCKDHA (branched-chain alpha-keto acid dehydrogenase E1 component alpha chain), also known as 2-oxoisovalerate dehydrogenase subunit alpha, belongs to the BCKDHA family. The branched-chain alpha-keto acid dehydrogenase complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). Recombinant human BCKDHA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molekulargewicht: 47.8 kDa (421aa) confirmed by MALDI-TOF

NCBI Accession: NP_000700