Pre-mRNA Branch Site Protein p14 (SF3B14) (AA 1-125) protein (His tag)

Produktnummer ABIN7676483

Dieses Recombinant Pre-mRNA Branch Site Protein p14-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für Pre-mRNA Branch Site Protein p14 (SF3B14) (AA 1-125) protein (His tag) (ABIN7676483)

Target: Pre-mRNA Branch Site Protein p14 (SF3B14)

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 95 %

Produktdetails

Proteineigenschaft: AA 1-125

Aufreinigungstag / Konjugat: His tag

Sequenz: MGSSHHHHHH SSGLVPRGSH MAMQAAKRAN IRLPPEVNRI LYIRNLPYKI TAEEMYDIFG KYGPIRQIRV GNTPETRGTA YVVYEDIFDA KNACDHLSGF NVCNRYLVVL YYNANRAFQK MDTKKKEEQL KLLKEKYGIN TDPPK

Produktmerkmale: SF3B14, 1-125aa, Human, His tag, E.coli

Aufreinigung: > 95% by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 0.2M NaCl, 5 mM DTT, 1 mM EDTA, 30 % glycerol

Handhabung: Avoid repeated freezing and thawing cycles

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: Pre-mRNA Branch Site Protein p14 (SF3B14)

Andere Bezeichnung: SF3B14A/SF3B6

Hintergrund: SF3B14, also known as SAP14, is a 125 amino acid nuclear protein that is a component of the splicing factor 3b complex. Splicing factor 3b associates with both the u2 and u11/u12 small nuclear ribonucleoprotein complexes (u2 snRNP) of spliceosomes. Required for the splicing of pre-mRNA, SF3B14 enters the spliceosome and associates with the pre-mRNA branch site facilitating the interaction of snRNP with the branch sites of u2 and u12 of the 17S u2 and the 18S u11/u12 snRNP complex. Recombinant human SF3B14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molekulargewicht: 16.7 kDa (145aa) confirmed by MALDI-TOF

NCBI Accession: NP_057131