3'(2'), 5'-Bisphosphate Nucleotidase 1 (BPNT1) protein (HIS)

Name: 3'(2'), 5'-Bisphosphate Nucleotidase 1 (BPNT1)

Synonyme: PIP, BPntase, Sal3, MGC93112, MGC82412, BPNT1, MGC126947

Hergestellt in: Escherichia Coli (E. coli)

Herkunft (Gen): Human

Konjugat: HIS

Applikation: SDS-PAGE (SDS)

Produktnummer: ABIN667334

Produktbeschreibung

Produktmerkmale: BPNT1, 1-308aa, Human, His tag, E.coli

Format: Liquid

Beschreibung: BPNT1, also known as BPntase, PAP phosphatase or PIP (PAP-inositol-1,4-phosphatase), is a member of the magnesium-dependent, lithium-sensitive phosphomono-esterase superfamily. Using magnesium as a cofactor, BPNT1 catalyzes the conversion of PAPS (adenosine 3'-phosphate 5' phosphosulfate) to APS (adenosine 5'-phosphosulfate) and the conversion of PAP (3'(2')-phosphoadenosine 5' phosphate) to AMP (adenosine 5'-phosphate). Expressed ubiquitously with highest levels in brain and kidney, BPNT1 is potently inhibited by lithium, a drug used for the treatment of manic depression and bipolar affective disorder, suggesting a possible role for BPNT1 in the etiology of mood disorders. Recombinant human BPNT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: 3'(2'), 5'-bisphosphate nucleotidase 1, PIP, PAP-inositol-1,4-phosphatase. NCBI no.: NP_006076

Molekulargewicht: 37.5 kDa (344aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Anwendungen

Konzentration: 0.5 mg/ml (determined by Bradford assay)

Reinheit: > 90 % by SDS – PAGE

Buffer: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 5mM DTT, 0.1M NaCl.

Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Bilder

3'(2'), 5'-Bisphosphate Nucleotidase 1 (BPNT1) protein (HIS)