NANS Protein (AA 1-359) (His tag)

Produktnummer ABIN667265

Dieses Recombinant NANS-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für NANS Protein (AA 1-359) (His tag) (ABIN667265)

Target: NANS (N-Acetylneuraminic Acid Synthase (NANS))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 95 %

Produktdetails

Proteineigenschaft: AA 1-359

Aufreinigungstag / Konjugat: Dieses NANS Protein ist gelabelt mit His tag.

Sequenz: MGSSHHHHHH SSGLVPRGSH MPLELELCPG RWVGGQHPCF IIAEIGQNHQ GDLDVAKRMI RMAKECGADC AKFQKSELEF KFNRKALERP YTSKHSWGKT YGEHKRHLEF SHDQYRELQR YAEEVGIFFT ASGMDEMAVE FLHELNVPFF KVGSGDTNNF PYLEKTAKKG RPMVISSGMQ SMDTMKQVYQ IVKPLNPNFC FLQCTSAYPL QPEDVNLRVI SEYQKLFPDI PIGYSGHETG IAISVAAVAL GAKVLERHIT LDKTWKGSDH SASLEPGELA ELVRSVRLVE RALGSPTKQL LPCEMACNEK LGKSVVAKVK IPEGTILTMD MLTVKVGEPK GYPPEDIFNL VGKKVLVTVE EDDTIMEELV DNHGKKIKS

Produktmerkmale: NANS, 1-359aa, Human, His tag, E.coli

Aufreinigung: > 95% by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 0.1M NaCl, 1 mM DTT, 10 % glycerol

Handhabung: Avoid repeated freezing and thawing cycles

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: NANS (N-Acetylneuraminic Acid Synthase (NANS))

Andere Bezeichnung: NANS

Hintergrund: NANS, also known as SAS, is a 359 amino acid protein that contains one AFP (antifreeze proteins) -like domain and functions in the biosynthesis of sialic acids. Expressed ubiquitously, NANS enzymatically catalyzes the H2O-dependent formation of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), both of which are sialic acids. NANS uses N-acetylmannosamine 6-phosphate as a substrate for Neu5Ac synthesis and mannose 6-phosphate as a substrate for KDN synthesis. Recombinant human NANS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molekulargewicht: 42.4 kDa (379aa) confirmed by MALDI-TOF

NCBI Accession: NP_061819