Adenylate Kinase 1 Protein (AK1) (AA 1-194) (His tag)

Produktnummer ABIN667254

Dieses Recombinant Adenylate Kinase 1-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für Adenylate Kinase 1 Protein (AK1) (AA 1-194) (His tag) (ABIN667254)

Target: Adenylate Kinase 1 (AK1)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Reinheit: > 95 %

Produktdetails

Proteineigenschaft: AA 1-194

Aufreinigungstag / Konjugat: Dieses Adenylate Kinase 1 Protein ist gelabelt mit His tag.

Sequenz: MGSSHHHHHH SSGLVPRGSH MEEKLKKTKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRSEVSSG SARGKKLSEI MEKGQLVPLE TVLDMLRDAM VAKVNTSKGF LIDGYPREVQ QGEEFERRIG QPTLLLYVDA GPETMTQRLL KRGETSGRVD DNEETIKKRL ETYYKATEPV IAFYEKRGIV RKVNAEGSVD SVFSQVCTHL DALK

Produktmerkmale: AK1, 1-194aa, Human, His tag, E.coli

Aufreinigung: > 95% by SDS-PAGE

Biological Activity Comment: Specific activity: > 600unit/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 7.5) containing 10 % glycerol

Handhabung: Avoid repeated freezing and thawing cycles

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: Adenylate Kinase 1 (AK1)

Andere Bezeichnung: AK1

Hintergrund: AK1 is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and erythrocytes. It is a small ubiquitous enzyme which is essential for maintenance and cell growth. Defects in AK1 are the cause of a form of hemolytic anemia. Recombinant human AK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Molekulargewicht: 23.7 kDa (214aa) confirmed by MALDI-TOF

NCBI Accession: NP_000467

Pathways: Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process