N-Acylneuraminate-9-Phosphatase (NANP) protein (HIS)

Name: N-Acylneuraminate-9-Phosphatase (NANP)

Synonyme: HDHD4, MGC26833, C20orf147, dJ694B14.3, Hdhd4, MGC103377, 1600031M04Rik, A130094D17Rik, MGC105812, RGD1306009

Hergestellt in: Escherichia Coli (E. coli)

Herkunft (Gen): Human

Konjugat: HIS

Applikation: SDS-PAGE (SDS)

Produktnummer: ABIN667232

Produktbeschreibung

Produktmerkmale: NANP, 1-248aa, Human, His tag, E.coli

Format: Liquid

Beschreibung: NANP (N-acylneuraminate-9-phosphatase), also known as HDHD4 (Haloacid dehalogenase-like hydrolase domain-containing protein 4), is belongs to the haloacid dehalogenase (HAD) family and is responsible for dephosphorylating N-acylneuraminate 9-phosphate to form N-acylneuraminate (N-acylneuraminate 9-phosphate + H2O = N-acylneuraminate + phosphate). Characteristic of the HAD phosphatase family, the catalytic activity of NANP is dependent upon the presence of magnesium and is inhibited by vanadate and calcium. Recombinant human NANP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: HDHD4, C20orf147, Neu5Ac-9-Pase, N-acylneuraminate-9-phosphatase. NCBI no.: NP_689880

Molekulargewicht: 31.9kDa (284aa), confirmed by MALDI-TOF

Anwendungen

Konzentration: 0.5mg/ml (determined by Bradford assay)

Reinheit: > 90% by SDS - PAGE

Buffer: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 2mM DTT, 100mM NaCl

Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Bilder

N-Acylneuraminate-9-Phosphatase (NANP) protein (HIS)