Selenoprotein (MSRB1) (AA 1-116) protein (His tag)

Produktnummer ABIN667227

Dieses Recombinant Selenoprotein-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für Selenoprotein (MSRB1) (AA 1-116) protein (His tag) (ABIN667227)

Target: Selenoprotein (MSRB1)

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 90 %

Produktdetails

Proteineigenschaft: AA 1-116

Aufreinigungstag / Konjugat: His tag

Sequenz: MSFCSFFGGE VFQNHFEPGV YVCAKCGYEL FSSRSKYAHS SPWPAFTETI HADSVAKRPE HNRSEALKVS CGKCGNGLGH EFLNDGPKPG QSRFCIFSSS LKFVPKGKET SASQGH

Produktmerkmale: SEPX1, 1-116aa, Human, His tag, E.coli

Aufreinigung: > 90% by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 7.5) containing 1 mM DTT, 0.1 mM PMSF, 2 mM EDTA, 10 % glycerol

Handhabung: Avoid repeated freezing and thawing cycles

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: Selenoprotein (MSRB1)

Andere Bezeichnung: Seleno R/MSRB1

Hintergrund: SEPX1, also known as Methionine sulfoxide reductase B1 (MSRB1), is a selenoprotein. Methionine sulfoxide reductases (MSRs) catalyze reduction of free and protein-bound methionine sulfoxides to corresponding methionines. The oxidation of methionine by ROS generates a diastereomeric mixture of methionine-S-sulfoxide (Met-S-SO) and methionine-R-sulfoxide (Met-R-SO). Two distinct enzyme families evolved for reduction of these sulfoxides, with methionine-S-sulfoxide reductase (MsrA) being stereospecific for Met-S-SO and methionine-R-sulfoxide reductase (MsrB) for Met-R-SO. In bacteria, the selenocystein (Sec/u) element is located immediately following the uGA codon within the reading frame for the selenoprotein so we mutated Sec-95 to Cys. Recombinant human SEPX1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Molekulargewicht: 14.8 kDa (136aa) confirmed by MALDI-TOF

NCBI Accession: NP_057416