HARS protein (HIS)

Name: HARS

Hergestellt in: Escherichia Coli (E. coli)

Herkunft (Gen): Human

Konjugat: HIS

Applikation: SDS-PAGE (SDS)

Produktnummer: ABIN667221

Produktbeschreibung

Produktmerkmale: HARS, 1-509aa, Human, His tag, E.coli

Format: Liquid

Beschreibung: HARS, also known as Histidyl-tRNA synthetase, function to catalyze the aminoacylation of tRNAs by their corresponding amino acids. HARS is from the class II family of aminoacyl-tRNA synthetases. It is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. HARS is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Recombinant human HARS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: FLJ20491, HRS, HisRS, Histidine--tRNA ligase, Histidyl-tRNA synthetase, cytoplasmic. NCBI no.: NP_002100

Molekulargewicht: 59.4 kDa (532aa), confirmed by MALDI-TOF

Anwendungen

Konzentration: 1 mg/ml (determined by Bradford assay)

Reinheit: > 95 % by SDS – PAGE

Buffer: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.

Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Bilder

HARS protein (HIS)