ARF1 Protein (AA 1-181) (His tag)

Produktnummer ABIN667162

Recombinant ARF1-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für ARF1 Protein (AA 1-181) (His tag) (ABIN667162)

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 90 %

Produktdetails

Proteineigenschaft: AA 1-181

Aufreinigungstag / Konjugat: Dieses ARF1 Protein ist gelabelt mit His tag.

Sequenz: MGNIFANLFK GLFGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV NEAREELMRM LAEDELRDAV LLVFANKQDL PNAMNAAEIT DKLGLHSLRH RNWYIQATCA TSGDGLYEGL DWLSNQLRNQ K

Produktmerkmale: ARF1, 1-181aa, Human, His tag, E.coli

Aufreinigung: > 90% by SDS-PAGE

Endotoxin-Niveau: < 1 EU per 1ug of protein (determined by LAL method)

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol 0.1M NaCl,1 mM DTT

Handhabung: Avoid repeated freezing and thawing cycles

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Andere Bezeichnung: ARF1

Hintergrund: ARF1, also known as ADP-ribosylation factor 1, is a small guanine nucleotide-binding protein that enhances the enzymatic activities of cholera toxin. It is essential and ubiquitous in eukaryotes, as this protein is involved in vesicular transport and functioning via phospholipase D activation. ARF1 plays a role in membrane traffic and organelle integrity and are intimately tied to their reversible association with membranes and distinct interactions with membrane phospholipids. Recombinant human ARF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molekulargewicht: 22.8 kDa (201aa) confirmed by MALDI-TOF

NCBI Accession: NP_001649

Pathways: Transition Metal Ion Homeostasis, Inositol Metabolic Process