PFN2 Protein (AA 1-140) (His tag)
Kurzübersicht für PFN2 Protein (AA 1-140) (His tag) (ABIN667130)
Target
Alle PFN2 Proteine anzeigenProtein-Typ
Spezies
Quelle
Applikation
Reinheit
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Proteineigenschaft
- AA 1-140
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Aufreinigungstag / Konjugat
- Dieses PFN2 Protein ist gelabelt mit His tag.
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Sequenz
- MAGWQSYVDN LMCDGCCQEA AIVGYCDAKY VWAATAGGVF QSITPIEIDM IVGKDREGFF TNGLALGAKK CSVIRDSLYV DGDCTMDIRT KSQGGEPTYN VAVGRAGRVL VFVMGKEGVH GGGLNKKAYS MAKYLRDSGF
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Produktmerkmale
- PFN2, 1-140 aa, Human, His-tagged, Recombinant, E.coli
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Aufreinigung
- > 95% by SDS-PAGE
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Profilin 2 (PFN2) (AA 1-140) protein (GST tag)
PFN2 Spezies: Human Wirt: Wheat germ Recombinant ELISA, WB, AP, AA
Profilin 2 (PFN2) (AA 1-140) protein (His-IF2DI Tag)PFN2 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Profilin 2 (PFN2) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)PFN2 Spezies: Human Wirt: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol 1 mM DTT
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Handhabung
- Avoid repeated freezing and thawing cycles
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- PFN2 (Profilin 2 (PFN2))
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Andere Bezeichnung
- Profilin 2/PFN2
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Hintergrund
- PFN2 is a ubiquitous actin monomer-binding protein belonging to the profilin family. This protein is ubiquitously expressed with highest expression in kidney, brain and skeletal muscle. Like other members of the profilin family, PFN2 functions as an actin monomer-binding protein that influences the structure of the cytoskeleton by regulating actin polymerization in response to extracellular signals. At high concentrations, it prevents the polymerization of actin whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Recombinant PFN2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molekulargewicht
- 17.2 kDa (160aa) confirmed by MALDI-TOF
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Pathways
- Regulation of Actin Filament Polymerization, Synaptic Vesicle Exocytosis
Target
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