DCMP Deaminase (DCTD) (AA 1-178) protein (His tag)
Kurzübersicht für DCMP Deaminase (DCTD) (AA 1-178) protein (His tag) (ABIN667034)
Target
Alle DCMP Deaminase (DCTD) Proteine anzeigenProtein-Typ
Spezies
Quelle
Applikation
Reinheit
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Proteineigenschaft
- AA 1-178
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Aufreinigungstag / Konjugat
- His tag
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Sequenz
- MGSSHHHHHH SSGLVPRGSH MSEVSCKKRD DYLEWPEYFM AVAFLSAQRS KDPNSQVGAC IVNSENKIVG IGYNGMPNGC SDDVLPWRRT AENKLDTKYP YVCHAELNAI MNKNSTDVKG CSMYVALFPC NECAKLIIQA GIKEVIFMSD KYHDSDEATA ARLLFNMAGV TFRKFIPKCS KIVIDFDSIN SRPSQKLQ
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Produktmerkmale
- DCTD, 1-178aa, Human, His tag, E.coli
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Aufreinigung
- > 90% by SDS-PAGE
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DCMP Deaminase (DCTD) (AA 1-178) protein (His tag)
custom-made DCTD Spezies: Human Wirt: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
DCMP Deaminase (DCTD) (AA 1-178) protein (His tag)custom-made DCTD Spezies: Maus Wirt: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
DCMP Deaminase (DCTD) (AA 1-189) protein (GST tag)DCTD Spezies: Human Wirt: Wheat germ Recombinant ELISA, WB, AA, AP
DCMP Deaminase (DCTD) (AA 1-178) protein (His-IF2DI Tag)DCTD Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
DCMP Deaminase (DCTD) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)DCTD Spezies: Human Wirt: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
DCMP Deaminase (DCTD) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)DCTD Spezies: Human Wirt: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 0.5 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 0.1M NaCl, 1 mM DTT, 20 % glycerol
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Handhabung
- Avoid repeated freezing and thawing cycles
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- DCMP Deaminase (DCTD)
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Andere Bezeichnung
- DCTD
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Hintergrund
- DCTD (deoxycytidylate deaminase), also known as dCMP deaminase, is a 178 amino acid allosteric enzyme that exists as a homohexamer and belongs to the cytidine and deoxycytidylate deaminase protein family. using zinc as a cofactor, DCTD catalyzes the deamination of dCMP to duMP, thereby producing the nucleotide substrate (duMP) that is used by thymidylate synthase. Recombinant human DCTD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques
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Molekulargewicht
- 22.1 kDa (198aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_001912
Target
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