DDT Protein (AA 1-118) (His tag)
Kurzübersicht für DDT Protein (AA 1-118) (His tag) (ABIN667020)
Target
Alle DDT Proteine anzeigenProtein-Typ
Spezies
Quelle
Applikation
Reinheit
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Proteineigenschaft
- AA 1-118
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Aufreinigungstag / Konjugat
- Dieses DDT Protein ist gelabelt mit His tag.
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Sequenz
- MPFLELDTNL PANRVPAGLE KRLCAAAASI LGKPADRVNV TVRPGLAMAL SGSTEPCAQL SISSIGVVGT AEDNRSHSAH FFEFLTKELA LGQDRILIRF FPLESWQIGK IGTVMTFL
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Produktmerkmale
- DDT, 1-118aa, Human, His tag, E.coli
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Aufreinigung
- > 95% by SDS-PAGE
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Endotoxin-Niveau
- < 1 EU per 1ug of protein (determined by LAL method)
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol
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Handhabung
- Avoid repeated freezing and thawing cycles
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- DDT (D-Dopachrome Tautomerase (DDT))
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Andere Bezeichnung
- DDT
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Hintergrund
- DDT is an enzyme that catayzes the tautomerization of D-dopachrome to give 5, 6-dihydroxyindole (DHI). This protein belongs to the family of lyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds. It shares a homologous amino acid sequence (33 % identical) with the macrophage migration inhibitory factor (MIF) and possesses similar tautomerase activity. In addition, it may functions a proinflammatory cytokine. Recombinant human DDT, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
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Molekulargewicht
- 14.8 kDa (138aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_001346
Target
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