FKBP1A Protein (AA 1-108) (His tag)

Produktnummer ABIN666754

Recombinant FKBP1A-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für FKBP1A Protein (AA 1-108) (His tag) (ABIN666754)

Target: FKBP1A (FK506 Binding Protein 1A, 12kDa (FKBP1A))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Reinheit: > 95 %

Produktdetails

Proteineigenschaft: AA 1-108

Aufreinigungstag / Konjugat: Dieses FKBP1A Protein ist gelabelt mit His tag.

Sequenz: MGVQVETISP GDGRTFPKRG QTCVVHYTGM LEDGKKFDSS RDRNKPFKFM LGKQEVIRGW EEGVAQMSVG QRAKLTISPD YAYGATGHPG IIPPHATLVF DVELLKLE

Produktmerkmale: FKBP1a /FKBP12, 1-108aa, Human, His-tagged, Recombinant, E.coli

Aufreinigung: > 95% by SDS-PAGE

Endotoxin-Niveau: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 800nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 100 mM NaCl, 1 mM DTT, 10 % glycerol

Handhabung: Avoid repeated freezing and thawing cycles

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: FKBP1A (FK506 Binding Protein 1A, 12kDa (FKBP1A))

Andere Bezeichnung: FKBP12/FKBP1A

Hintergrund: FK506 binding protein 1a, also known as FKBP12, belongs to the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP12 also plays a role in intracellular calcium regulation by associating with three types of calcium release channel complexes, cardiac and skeletal ryanodine receptors and the inositol 1, 4, 5-trisphosphate receptor. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. Recombinant human FKBP12, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Molekulargewicht: 14.1 kDa (128aa) confirmed by MALDI-TOF

NCBI Accession: NP_463460

Pathways: Negative Regulation of Transporter Activity, Methionine Biosynthetic Process