ACP1 Protein (AA 1-158) (His tag)

Produktnummer ABIN666750

Dieses Recombinant ACP1-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für ACP1 Protein (AA 1-158) (His tag) (ABIN666750)

Target: ACP1 (Acid Phosphatase 1, Soluble (ACP1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Reinheit: > 95 %

Produktdetails

Proteineigenschaft: AA 1-158

Aufreinigungstag / Konjugat: Dieses ACP1 Protein ist gelabelt mit His tag.

Sequenz: MAEQATKSVL FVCLGNICRS PIAEAVFRKL VTDQNISENW VIDSGAVSDW NVGRSPDPRA VSCLRNHGIH TAHKARQITK EDFATFDYIL CMDESNLRDL NRKSNQVKTC KAKIELLGSY DPQKQLIIED PYYGNDSDFE TVYQQCVRCC RAFLEKAH

Produktmerkmale: Acid phosphatase 1,1-158aa, Human, His-tagged, Recombinant, E.coli

Aufreinigung: > 95% by SDS-PAGE

Endotoxin-Niveau: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 15,000unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 20 mM MES buffer ( pH 6.0) containing 0.1 mM PMSF, 2 mM EDTA, 10 % glycerol

Handhabung: Avoid repeated freezing and thawing cycles

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: ACP1 (Acid Phosphatase 1, Soluble (ACP1))

Andere Bezeichnung: LMW-PTP/ACP1

Hintergrund: Acid phosphatase 1, soluble, also known as ACP1, belongs to the phosphotyrosine protein. It functions as an acid phosphatase and a protein tyrosine phosphatase (PTPase) present in all human tissues, including adipocytes. This enzyme hydrolyzes protein tyrosine phosphate to protein tyrosine and orthophosphate, and also orthophosphoric monoesters to alcohol and orthophosphate. Recombinant ACP1, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography techniques.

Molekulargewicht: 20.1 kDa (178aa) confirmed by MALDI-TOF

NCBI Accession: NP_009030