PPM1A Protein (AA 1-382) (His tag)

Produktnummer ABIN666675

Dieses Recombinant PPM1A-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für PPM1A Protein (AA 1-382) (His tag) (ABIN666675)

Target: PPM1A (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A (PPM1A))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Reinheit: > 95 %

Produktdetails

Proteineigenschaft: AA 1-382

Aufreinigungstag / Konjugat: Dieses PPM1A Protein ist gelabelt mit His tag.

Sequenz: MGAF LDKPKMEKHN AQGQGNGLRY GLSSMQGWRV EMEDAHTAVI GLPSGLESWS FFAVYDGHAG SQVAKYCCEH LLDHITNNQD FKGSAGAPSV ENVKNGIRTG FLEIDEHMRV MSEKKHGADR SGSTAVGVLI SPQHTYFINC GDSRGLLCRN RKVHFFTQDH KPSNPLEKER IQNAGGSVMI QRVNGSLAVS RALGDFDYKC VHGKGPTEQL VSPEPEVHDI ERSEEDDQFI ILACDGIWDV MGNEELCDFV RSRLEVTDDL EKVCNEVVDT CLYKGSRDNM SVILICFPNA PKVSPEAVKK EAELDKYLEC RVEEIIKKQG EGVPDLVHVM RTLASENIPS LPPGGELASK RNVIEAVYNR LNPYKNDDTD STSTDDMW

Produktmerkmale: His-PP2Calpha(His-tagging Protein phosphatase 2C), Human, Recombinant, E.coli

Aufreinigung: > 95% by SDS-PAGE

Endotoxin-Niveau: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 1,400unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 10 mM Tris-HCl buffer ( pH 7.5) containing 50 mM NaCl, 2 mM DTT, 1 mM MnCl2, 20 % glycerol

Handhabung: Avoid repeated freezing and thawing cycles

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: PPM1A (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A (PPM1A))

Andere Bezeichnung: PP2C alpha/PPM1A

Hintergrund: Protein phosphatase 2C (PP2C alpha) is a Mn2+- or Mg2+- dependent protein serine/threonine phosphatase that is essential for regulating cellular stress response in eukaryotes. The protein coding region of PP2C alpha (amino acids 1-382) was cloned into an E. coli expression vector. PP2C alpha was overexpressed in E. coli as a soluble His-tag fusion protein, and it was purified by conventional column chromatographic techniques.

Molekulargewicht: 46.6 kDa (418aa) confirmed by MALDI-TOF

NCBI Accession: NP_066283