NCALD Protein (AA 1-193) (His tag)
Kurzübersicht für NCALD Protein (AA 1-193) (His tag) (ABIN666638)
Target
Alle NCALD Proteine anzeigenProtein-Typ
Spezies
Quelle
Applikation
Reinheit
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Proteineigenschaft
- AA 1-193
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Aufreinigungstag / Konjugat
- Dieses NCALD Protein ist gelabelt mit His tag.
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Sequenz
- MGSSHHHHHH SSGLVPRGSH MGKQNSKLRP EVMQDLLEST DFTEHEIQEW YKGFLRDCPS GHLSMEEFKK IYGNFFPYGD ASKFAEHVFR TFDANGDGTI DFREFIIALS VTSRGKLEQK LKWAFSMYDL DGNGYISKAE MLEIVQAIYK MVSSVMKMPE DESTPEKRTE KIFRQMDTNR DGKLSLEEFI RGAKSDPSIV RLLQCDPSSA GQF
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Produktmerkmale
- NCALD, 1-193aa, Human, His tag, E.coli
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Aufreinigung
- > 90% by SDS-PAGE
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 0.5 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 40 % glycerol, 100 mM NaCl
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Handhabung
- Avoid repeated freezing and thawing cycles
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- NCALD (Neurocalcin delta (NCALD))
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Andere Bezeichnung
- NCALD
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Hintergrund
- Neurocalcin-delta, also known NCALD, is a member of the neuronal calcium sensor (NCS) family. NCALD is cytosolic at resting calcium levels, however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. This protein is thought to be a regulator of signal transduction by G protein-coupled receptor. Recombinant human NCALD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molekulargewicht
- 24.4 kDa (213aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_001035720
Target
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