Adenylate Kinase 2 Protein (AK2) (AA 1-239) (His tag)
Kurzübersicht für Adenylate Kinase 2 Protein (AK2) (AA 1-239) (His tag) (ABIN666627)
Target
Alle Adenylate Kinase 2 (AK2) Proteine anzeigenProtein-Typ
Biologische Aktivität
Spezies
Quelle
Applikation
Reinheit
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Proteineigenschaft
- AA 1-239
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Aufreinigungstag / Konjugat
- Dieses Adenylate Kinase 2 Protein ist gelabelt mit His tag.
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Sequenz
- MAPSVPAAEP EYPKGIRAVL LGPPGAGKGT QAPRLAENFC VCHLATGDML RAMVASGSEL GKKLKATMDA GKLVSDEMVV ELIEKNLETP LCKNGFLLDG FPRTVRQAEM LDDLMEKRKE KLDSVIEFSI PDSLLIRRIT GRLIHPKSGR SYHEEFNPPK EPMKDDITGE PLIRRSDDNE KALKIRLQAY HTQTTPLIEY YRKRGIHSAI DASQTPDVVF ASILAAFSKA TCKDLVMFI
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Produktmerkmale
- AK2, 1-239aa, Human,His-Tagged, Recombinant, E.coli
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Aufreinigung
- > 95% by SDS-PAGE
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Endotoxin-Niveau
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Specific activity is > 25unit/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.
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Adenylate Kinase 2 (AK2) (AA 1-239) protein (His tag)
custom-made AK2 Spezies: Maus Wirt: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Adenylate Kinase 2 (AK2) (AA 1-239) protein (GST tag)AK2 Spezies: Human Wirt: Wheat germ Recombinant ELISA, WB, AA, AP
Adenylate Kinase 2 (AK2) (AA 1-239) protein (His-IF2DI Tag)AK2 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Adenylate Kinase 2 (AK2) (AA 1-239) protein (His-IF2DI Tag)AK2 Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Adenylate Kinase 2 (AK2) (AA 1-239) (Active) protein (His tag)AK2 Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant > 95 % SDS, EAA Active
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1 mg/mL
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Buffer
- Phosphate-Buffered Saline ( pH 7.4) containing 2 mM DTT, 20 % glycerol
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Handhabung
- Avoid repeated freezing and thawing cycles
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- Adenylate Kinase 2 (AK2)
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Andere Bezeichnung
- Adenylate kinase 2
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Hintergrund
- Adenylate kinase (AK, adenosine triphosphate-adenosine monophosphate [ATP-AMP] phospho-transferase, EC 2. 7. 4. 3) is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Three isozymes (AK1, AK2 and AK3) are characterized in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Recombinant human AK2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molekulargewicht
- 28.6 kDa (259aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_001616
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Pathways
- Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process
Target
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