AK4 Protein (AA 1-223, C22S) (His tag)
Kurzübersicht für AK4 Protein (AA 1-223, C22S) (His tag) (ABIN666626)
Target
Alle AK4 Proteine anzeigenProtein-Typ
Spezies
Quelle
Applikation
Reinheit
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Proteineigenschaft
- AA 1-223, C22S
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Aufreinigungstag / Konjugat
- Dieses AK4 Protein ist gelabelt mit His tag.
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Sequenz
- MASK LLRAVILGPP GSGKGTVSQR IAQNFGLQHL SSGHFLRENI KASTEVGEMA KQYIEKSLLV PDHVITRLMM SELENRRGQH WLLDGFPRTL GQAEALDKIC EVDLVISLNI PFETLKDRLS RRWIHPPSGR VYNLDFNPPH VHGIDDVTGE PLVQQEDDKP EAVAARLRQY KDVAKPVIEL YKSRGVLHQF SGTETNKIWP YVYTLFSNKI TPIQSKEAY
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Produktmerkmale
- Adenylate kinase 3(C22S), 1- 223aa, Human, His-tagged, Recombinant, E.coli
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Aufreinigung
- > 90% by SDS-PAGE
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Adenylate Kinase 4 (AK4) (AA 1-223) protein (His tag)
custom-made AK4 Spezies: Maus Wirt: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Adenylate Kinase 4 (AK4) (AA 1-223) protein (His tag)custom-made AK4 Spezies: Human Wirt: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Adenylate Kinase 4 (AK4) (AA 1-223) protein (GST tag)AK4 Spezies: Human Wirt: Wheat germ Recombinant ELISA, WB, AA, AP
Adenylate Kinase 4 (AK4) protein (GST tag,His tag)AK4 Spezies: Human Wirt: Baculovirus infected Insect Cells Recombinant > 94 % as determined by reducing SDS-PAGE.
Adenylate Kinase 4 (AK4) (AA 1-223) protein (His tag)AK4 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 90 % SDS
Adenylate Kinase 4 (AK4) (AA 1-223) protein (His-IF2DI Tag)AK4 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.5) containing 2 mM DTT, 30 % glycerol, 0.1M NaCl
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Handhabung
- Avoid repeated freezing and thawing cycles
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- AK4 (Adenylate Kinase 4 (AK4))
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Andere Bezeichnung
- AK4
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Hintergrund
- Adenylate kinase (AK, adenosine triphosphate-adenosine monophosphate [ATP-AMP] phospho-transferase, EC 2. 7. 4. 3) is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Five isozymes of adenylate kinase have been identified in vertebrates. AK1 is present in the cytosol of skeletal muscle, brain, and erythrocyte, while AK2 is localized in the intermembrane space of mitochondria of liver, kidney, spleen and heart. AK3, called GTP:AMP phosphotransferase, exists in the mitochondrial matrix of liver and heart. These isozymes contribute to homeostasis of the adenine nucleotide composition in the cell. Recombinant human AK3, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molekulargewicht
- 29.3 kDa (259aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_982289
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Pathways
- Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process
Target
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