Immunoglobulin E (IgE) Protein

Name: Immunoglobulin E (IgE)

Hergestellt in: Human

Herkunft (Gen): Human

Applikation: ELISA, Western Blot (WB), Durchflusszytometrie (FACS), Funktionale Studien (Func)

Produktnummer: ABIN363700

Produktbeschreibung

Gen-ID: IGHE, chromosome 14q32.33

Herstellung: Source of protein: Human B cell hybridoma from healthy donors (CD40 cell culture system)

Format: Affinity-purified, Low-endotoxin, azide-free (LEAF)

Isotyp: IgE lambda

Klon: SUS11-NBS01

Beschreibung: Recombinant human IgE to be used as reference for IgE concentration measurements in biological samples (ELISA, FACS). IgE (SUS-11) recognizes the human high affinity IgE receptor (FceRIa), the human low affinity IgE receptor (CD23) and does not interact with the corresponding IgE receptors of other species. It can be used as functional reagent in mast cell / basophil cellular assays, e.g. for controlled sensitization of such cells and subsequent cross-linking with either anti-human IgE mAbs mAb LE27 or a specific recombinant cross-linking agent.

Spezifität: Binds with Fc effector domain to human and non human primate IgE receptors, no cross-reactivity with rodent or canine IgE receptors, specific antigen unknown

Anwendungen

Applikationshinweise: For functional cellular assays (mast cell / basophil activation), in particular when used at dilutions < 1:100 Quality assurance: Reactivity > 99%, compared to calibrator (Internal Standard mAb BSW17), tested by ELISA binding to immobilized human Standard IgE (SUS-11)

Konzentration: 1mg/ml

Reinheit: > 95%, tested by analytical Gel Permeation Chromatography

Reinigung: Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B

Buffer: PBS, pH=7.2, sterile filtered, contains no NaN3

Lagerung: Store undiluted at 4°C. For long term storage, aliquot and store at -20°C

Forschungsgebiet: Allergie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

product purity: gel permeation chromatography (Superose 12/HR)

Target binding kinetics (Biacore

binding of increasing amounts of IgE(SUS-11) to recombinant rat RBL2H3 mast cells expressing the human high affinity IgE receptor

Publikationen

Publikationen: Zuercher, Lang, Aebischer et al.: "IgE-producing hybridomas established after B-cell culture in the CD40 system." in: Immunology letters, Vol. 46, Issue 1-2, pp. 49-57, 1995 (PubMed).

Pachlopnik, Staempfli, Rudolf et al.: "Antigen interaction and heat inactivation expose new epitopes on human IgE." in: International archives of allergy and immunology, Vol. 117, Issue 4, pp. 231-8, 1999 (PubMed).