PHF8 Protein (DYKDDDDK Tag)

Produktnummer ABIN2669683

Dieses Recombinant PHF8-Protein wird in Baculovirus produziert.

Kurzübersicht für PHF8 Protein (DYKDDDDK Tag) (ABIN2669683)

Target: PHF8 (PHD Finger Protein 8 (PHF8))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Baculovirus

Applikation: Enzyme Activity Assay (EAA), Screening Assay (ScA)

Produktdetails

Aufreinigungstag / Konjugat: Dieses PHF8 Protein ist gelabelt mit DYKDDDDK Tag.

Produktmerkmale: Recombinant PHF8 (accession number NP_055922.1) was expressed in Sf9 and contains an N-terminal FLAG tag with a molecular weight of 121.1 kDa.

Anwendungsinformationen

Applikationshinweise: Recombinant PHF8 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3K9me2, H3K36me2 and H4K20me1 demethylase. Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02 % Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2•6H2O, 1 mM TCEP, 100 nM Recombinant PHF8 protein, and 3.3 μM H3K9me2 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Antigendetails

Target: PHF8 (PHD Finger Protein 8 (PHF8))

Andere Bezeichnung: PHF8

Hintergrund: PHF8 (PHD finger protein 8), also known as Lysine (K)-specific demethylase 7B (KDM7B) and JmjC domain-containing histone demethylation protein 1D-B (JHDM1DB), is a member of the JmjC-containing (Jumonji-C) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. PHF8 has two N-terminal domains, a PHD finger that binds trimethylated lysine 4 of histone H3 (H3K4me3) and a Jumonji domain that demethylates monomethylated H3 Lys9 (H3K9me1), Histone H3 dimethyl Lys9 (H3K9me2), Histone H3 dimethyl Lys27 (H3K27me2) (which are all modifications associated with transcriptional repression) and also Histone H3 dimethyl Lys36 (H3K36me2). PHF8 depletion in mammalian neuronal cells results in decreased expression of follistatin and increased methylation of H3K9 and H3K27. It has also been suggested to function in gene silencing during brain development.

Molekulargewicht: 121.1 kDa