Nitrogenase (Nifh) Protein

Name: Nitrogenase (Nifh)

Applikation: Western Blot (WB)

Produktnummer: ABIN190740

Produktbeschreibung

Beschreibung: Nitrogenase is involved in biological fixation of nitrogen to assimilable ammonia. This product

Anwendungen

Applikationshinweise: Standard curve: 3 loads are recommended (0.5, 2 and 4µl).For most applications a sample load of 0.2µg of chlorophyll will give a NifH signalin this range. positive control: a 2µl load per well is optimal for most chemiluminescent detectionsystems. This standard is stabilized and does not require heating before loading on the gel. Additional Information: Global antibodies are raised against highly conserved amino acid sequences inthe NifH protein. The NifH protein standard can therefore be used in combinationwith global anti-NifH antibodies to quantitate NifH from a wide range ofcyanobacterial species. Quantitative western blot: detailed method description.

Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar