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Applikationshinweise
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Standard curve: 3 loads are recommended (2.5 and 10 µl).For most applications a sample load of 0.2µg of chlorophyll will give a IsiA signalin this range. positive control:a 2µl load per well is optimal for most chemiluminescent detectionsystems. Additional Information: antibodies are raised against highly conserved amino acid sequences in theIsiAprotein. The IsiA protein standard can therefore be used in combination withanti-IsiA antibodies to quantitate IsiA from a range of cyanobacteria. Quantitative western blot: detailed method description.
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Lagerung
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store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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