HSV1/2 IgG ELISA Kit

Produktnummer ABIN459368

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human HSV1/2 IgG.

Kurzübersicht für HSV1/2 IgG ELISA Kit (ABIN459368)

Target: HSV1/2 IgG (Anti-Herpes Simplex Virus Type 1/2 IgG (HSV1/2 IgG))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Indirect ELISA

Applikation: ELISA

Proben: Serum, Plasma

Produktdetails HSV1/2 IgG ELISA Kit

Verwendungszweck: anti-HSV 1+2 IgG is a solid-phase immunoanalytical test.

Marke: ELISA-VIDITEST

Analytische Methode: Semi-Quantitative

Bestandteile:

• ELISA break-away strips in the handling frame coated with the specific antigen STRIPS Ag 1 x 12 pcs
• 1.3 mL Standard A = Negative control human serum, r.t.u. 1) ST A/NC 1 vial
• 2.0 mL Standard D = Calibrator (human serum), r.t.u. ST D/CAL 1 vial
• 1.3 mL Standard E = Positive control human serum, r.t.u. ST E/PC 1 vial
• 13 mL Anti-human IgG animal antibodies labelled with horseradish peroxidase (anti-IgG Px conjugate) r.t.u. CONJ 1 vial
• 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate TMB, r.t.u. (TMB/H2O2) TMB 1 vial
• 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
• Instruction manual
• Quality Control Certificate
• 1) r.t.u., ready to use

Benötigtes Material:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll: anti-HSV 1+2 IgG is a solid-phase immunoanalytical test. The polystyrene strips are coated with a mixture of antigens, which bear immunodominant epitopes of HSV 1 and HSV 2. Anti-HSV antibodies from samples bind to the immobilized antigens. The other serum antibodies do not bind and are washed away those that formed complexes with the antigens are later on recognized by animal anti-human IgG antibodies labelled with horseradish peroxidase. The presence of the labelled antibodies is revealed by an enzymatic reaction with a chromogenic substrate. Negative samples do not react and the mild change in colour, if present, may be attributed to the reaction background.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu HSV1/2 IgG

Target: HSV1/2 IgG (Anti-Herpes Simplex Virus Type 1/2 IgG (HSV1/2 IgG))

Substanzklasse: Antibody