TNFSF8 ELISA Kit

Produktnummer ABIN456421

Human TNFSF8 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human TNFSF8.

Kurzübersicht für TNFSF8 ELISA Kit (ABIN456421)

Target: TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))

Bindungsspezifität: Soluble

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156-10 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma

Produktdetails TNFSF8 ELISA Kit

Untere Nachweisgrenze: 0.156 ng/mL

Verwendungszweck: This immunoassay kit allows for the specific measurement of human sCD30L concentrations in cell culture supernates, serum, and plasma.

Analytische Methode: Quantitative

Spezifität: This assay recognizes recombinant and natural human sCD30L.

Kreuzreaktivität (Details): No significant cross-reactivity or interference was observed.

Produktmerkmale: Homo sapiens,TNFSF8,Tumor necrosis factor ligand superfamily member 8,CD30 ligand,CD30-L,TNFSF8,CD30L,CD30LG,CD153,CD153

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for sCD30L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sCD30L present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for sCD30L is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sCD30L bound in the initial step. The color development is stopped and the intensity of the color is measured.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Details zu TNFSF8

Target: TNFSF8 (Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))

Andere Bezeichnung: TNFSF8

Hintergrund: The human CD30 ligand, CD153, is a 40kDa Type II transmembrane glycoprotein. It interacts with its receptor, CD30, as a homotrimer Soluble trimeric CD153 binds to membrane-anchored CD30 with a relatively high affinity and is very effective in triggering cell death and TNFα. CD153 is a member of the TNF superfamily which is primarily expressed by activated T cells, B cells and monocytes. Its function in programmed cell death has been proven, as well as its involvement in disorders like a variety of lymphoid malignancies like Hodgkin’s disease and anaplastic large cell lymphoma.