anti-b2 adrenergic Receptor Antibodies (IgG) ELISA Kit

Antigen: Anti-B2 Adrenergic Receptor Antibodies

Produktnummer: ABIN454022

Produktbeschreibung

Weitere Bezeichnung: anti-b2 adrenergic Receptor Antibodies (IgG)

Beschreibung: The Alpha-2 adrenergic receptor is a G protein-coupled receptor. ß receptors have the subtypes ß1, ß2 and ß3. Agonist binding causes a rise in the intracellular concentration of the second messenger cAMP. The anti-alpha2-adrenergic-receptor-antibody-EIA is designed for the determination of Antibodies (IgG) against the Alpha2-adrenergic-receptor in serum and plasma.

Sensitivität: 5 standards between 2,5 U/ml and 40 U/mlcut off:18 U/ml Sample materials:Serum, Plasma Intraassay-Precision:7.34% Interassay-Precision:10.82%

Anwendungen

Prinzip: The anti-alpha2-adrenergic-receptor-antibody-EIA is an antibody screening test. The alpha2-adrenergic-receptor has been pre-coated onto a microtiter plate. During the first incubation the anti-alpha2-adrenergic-receptor-antibodies of the samples are immobilised on the plate. The auto-antibodies are detected with a POD labeled anti-human IgG antibody. In the following enzymatic substrate reaction the intensity of the colour correlates with the concentration and/ or avidity of anti-alpha2-adrenergic-receptor-antibodies.

Protokoll: Incubation of samples/ standards/ controls 100 µl 120 min, 4°C. Wash Incubation of detection antibody 100 µl 1 hr, room temperature. Wash Substrate incubation 100 µl/well 20 min, room temperature. Add Stopp solution 100 µl/well. Read at 450 nm

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.