Chemokine (C-X-C Motif) Ligand 10 (CXCL10) ELISA Kit

Antigen: Chemokine (C-X-C Motif) Ligand 10 (CXCL10)

Synonyme: C7, IFI10, INP10, IP-10, crg-2, mob-1, SCYB10, gIP-10, IP10, CRG-2, Ifi10, Scyb10, CXCL10, MGC137238

Reaktivität: Maus

Applikation: ELISA

Produktnummer: ABIN411396

Produktbeschreibung

Produktmerkmale: For quantitative detection of mouse CXCL10 in sera, plasma, body fluids, tissue lysates or cell culture supernates.

Weitere Bezeichnung: C-X-C Motif Chemokine 10 (CXCL10)

Beschreibung: Chemokine (C-X-C motif) ligand 10 (CXCL10) or IP-10 is a small cytokine belonging to the CXC chemokine family that is also known as 10 kDa interferon-gamma-induced protein (?-IP10 or IP-10). CXCL10 is secreted by several cell types in response to IFN-?. These cell types include monocytes, endothelial cells and fibroblasts.1 CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis.2, 3 It is a potent inhibitor of angiogenesis in vivo.4 The gene for CXCL10 is located on human chromosome 4 in a cluster among several other CXC chemokines.5 It is a RAS target gene and is overexpressed in the majority of colorectal cancers.6 This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.7

Spezifität: No detectable cross-reactivity with any other cytokine.

Sensitivität: Sensitivity: < 1pg/ml. Range: 31.2pg/ml-2000pg/ml

Anwendungen

Prinzip: This mouse CXCL10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse CXCL10 specific-specific polyclonal antibodies were precoated onto 96-well plates. The mouse specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CXCL10 amount of sample captured in plate.

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing. Don

Bestandteile: Lyophilized recombinant mouse CXCL10 standard: 10ng/tube

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl, 450

Lagerung: Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) Four months at 4°C and eight months at -20°C.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Chemokine (C-X-C Motif) Ligand 10 (CXCL10) ELISA Kit