TAF4 Antikörper (AA 454-565)

Produktnummer ABIN968728

Dieser Maus Monoklonal Antikörper detektiert spezifisch TAF4 in WB und IF. Es zeigt Reaktivität gegenüber Proben von Human und Maus. Es wurde in 4+ Publikationen zitiert.

Kurzübersicht für TAF4 Antikörper (AA 454-565) (ABIN968728)

Target: TAF4 (TAF4 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 135kDa (TAF4))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TAF4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: 22-TAF[II]135

Produktdetails anti-TAF4 Antikörper

Bindungsspezifität: AA 454-565

Kreuzreaktivität: Maus

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human TAF[II]135 aa. 454-565

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-TAF4 Antikörper (ABIN968728)

Freiman, Albright, Chu, Zheng, Liang, Sha, Tjian: "Redundant role of tissue-selective TAF(II)105 in B lymphocytes." in: Molecular and cellular biology, Vol. 22, Issue 18, pp. 6564-72, (2002) (PubMed).

Shimohata, Nakajima, Yamada, Uchida, Onodera, Naruse, Kimura, Koide, Nozaki, Sano, Ishiguro, Sakoe, Ooshima, Sato, Ikeuchi, Oyake, Sato, Aoyagi, Hozumi, Nagatsu, Takiyama, Nishizawa, Goto, Kanazawa et al.: "Expanded polyglutamine stretches interact with TAFII130, interfering with CREB-dependent transcription. ..." in: Nature genetics, Vol. 26, Issue 1, pp. 29-36, (2000) (PubMed).

Saluja, Vassallo, Tanese: "Distinct subdomains of human TAFII130 are required for interactions with glutamine-rich transcriptional activators." in: Molecular and cellular biology, Vol. 18, Issue 10, pp. 5734-43, (1998) (PubMed).

Mengus, May, Carré, Chambon, Davidson: "Human TAF(II)135 potentiates transcriptional activation by the AF-2s of the retinoic acid, vitamin D3, and thyroid hormone receptors in mammalian cells." in: Genes & development, Vol. 11, Issue 11, pp. 1381-95, (1997) (PubMed).

Details zu TAF4

Target: TAF4 (TAF4 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 135kDa (TAF4))

Andere Bezeichnung: TAF II135

Hintergrund: Tightly associated factors (TAFs) play an essential role in transcriptional activation through their interaction with a variety of activators. TAF[II]135 (a.k.a. TAF[II]130) is a human homologue of Drosophila TAF[II]110, the first TAF shown to have coactivator activity. TAF[II]135 contains multiple glutamine-rich regions, as well as a coactivator domain (CAD). The glutamine-rich regions of TAF[II]135 facilitate interaction with Sp1 and CREB, which leads to enhancement of both Sp1- and CREB-mediated transcription. In addition, TAF[II]135 can potentiate transcriptional stimulation by AF-2 of the retinoic acid, thyroid hormone, and vitamine D3 receptors. However, TAF[II]135 does not interact with the AF-2s of the estrogen and retinoid X receptors. Interestingly, TAF[II]135 enhancement of CREB transcriptional activity may be disrupted by expanded polyglutamine (CAG) repeats found in at least eight different neurodegenerative disorders. Thus, TAF[II]135 may have important coactivator activities in many different transcription-regulating cell signaling pathways, and interference of TAF[II]135 activity by CAG repeats may be a common mechanism of specific types of neuropathologies.

Molekulargewicht: 135 kDa