gamma 1 Adaptin Antikörper (AA 642-821)

Produktnummer ABIN967934

Es sind 5+ Publikationen für dieses Produkt verfügbar. Der Maus Monoklonal anti-gamma 1 Adaptin Antikörper wird verwendet zum Nachweis von gamma 1 Adaptin in Proben von Human, Maus, Ratte und Hund. Er wurde validiert für WB, IHC, IP und IF.

Kurzübersicht für gamma 1 Adaptin Antikörper (AA 642-821) (ABIN967934)

Target: gamma 1 Adaptin (AP1G1) (Adaptor-Related Protein Complex 1, gamma 1 Subunit (AP1G1))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser gamma 1 Adaptin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Klon: 88-Adaptin gamma

Produktdetails anti-gamma 1 Adaptin Antikörper

Bindungsspezifität: AA 642-821

Kreuzreaktivität: Ratte (Rattus), Human, Hund

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Adaptin gamma aa. 642-821

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968539, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-gamma 1 Adaptin Antikörper (ABIN967934)

Nagahama, Suzuki, Hamada, Hatsuzawa, Tani, Yamamoto, Tagaya: "SVIP is a novel VCP/p97-interacting protein whose expression causes cell vacuolation." in: Molecular biology of the cell, Vol. 14, Issue 1, pp. 262-73, (2003) (PubMed).

Eskelinen, Illert, Tanaka, Schwarzmann, Blanz, Von Figura, Saftig: "Role of LAMP-2 in lysosome biogenesis and autophagy." in: Molecular biology of the cell, Vol. 13, Issue 9, pp. 3355-68, (2002) (PubMed).

Kalthoff, Groos, Kohl, Mahrhold, Ungewickell: "Clint: a novel clathrin-binding ENTH-domain protein at the Golgi." in: Molecular biology of the cell, Vol. 13, Issue 11, pp. 4060-73, (2002) (PubMed).

Wasiak, Legendre-Guillemin, Puertollano, Blondeau, Girard, de Heuvel, Boismenu, Bell, Bonifacino, McPherson: "Enthoprotin: a novel clathrin-associated protein identified through subcellular proteomics." in: The Journal of cell biology, Vol. 158, Issue 5, pp. 855-62, (2002) (PubMed).

Robinson: "Cloning and expression of gamma-adaptin, a component of clathrin-coated vesicles associated with the Golgi apparatus." in: The Journal of cell biology, Vol. 111, Issue 6 Pt 1, pp. 2319-26, (1991) (PubMed).

Details zu gamma 1 Adaptin

Target: gamma 1 Adaptin (AP1G1) (Adaptor-Related Protein Complex 1, gamma 1 Subunit (AP1G1))

Andere Bezeichnung: Adaptin gamma

Hintergrund: Sorting of integral membrane proteins at various stages of the endocytic and secretory pathways is mediated by vesicular trafficking between a variety of organelles. Two sorting signals are tyrosine-based and dileucine-based signals that interact with heterotetrameric adaptor protein complexes (AP-1, AP-2, AP-3, and AP-4), which are associated with the vesicle coats. These coatomers contain two large Adaptin proteins (gamma, alpha, delta, or epsilon and beta1, beta2, beta3, or beta4, respectively) that are noncovalently linked to one medium chain (µ1, µ2, µ3, or µ4) and one small chain ( sigma1, sigma2, sigma3, or sigma4). The AP-1 and AP-3 complexes are involved in protein sorting from the TGN and endosomes, while AP-2 adaptor complexes are involved in clathrin-mediated endocytosis. Adaptin gamma shows more homology with Adaptin alpha than with Adaptin beta. The conserved regions between Adaptins gamma and alpha could be important for binding to other components of the AP-1 and AP-2 complexes, respectively.

Molekulargewicht: 104 kDa