DNM2 Antikörper (AA 274-555)

Produktnummer ABIN967865

Der Maus Monoklonal Anti-DNM2-Antikörper wurde für WB, IHC, IF und IP validiert. Er ist geeignet, DNM2 in Proben von Human, Maus und Ratte zu detektieren. Es sind 5+ Publikationen verfügbar.

Kurzübersicht für DNM2 Antikörper (AA 274-555) (ABIN967865)

Target: DNM2 (Dynamin 2 (DNM2))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser DNM2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: 27-Dynamin II

Produktdetails anti-DNM2 Antikörper

Bindungsspezifität: AA 274-555

Kreuzreaktivität: Human, Maus

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat Dynamin II aa. 274-555

Isotyp: IgG2a

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389, ABIN968535

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-DNM2 Antikörper (ABIN967865)

Kessels, Engqvist-Goldstein, Drubin: "Association of mouse actin-binding protein 1 (mAbp1/SH3P7), an Src kinase target, with dynamic regions of the cortical actin cytoskeleton in response to Rac1 activation." in: Molecular biology of the cell, Vol. 11, Issue 1, pp. 393-412, (2000) (PubMed).

Kranenburg, Verlaan, Moolenaar: "Dynamin is required for the activation of mitogen-activated protein (MAP) kinase by MAP kinase kinase." in: The Journal of biological chemistry, Vol. 274, Issue 50, pp. 35301-4, (2000) (PubMed).

McNiven, Kim, Krueger, Orth, Cao, Wong: "Regulated interactions between dynamin and the actin-binding protein cortactin modulate cell shape." in: The Journal of cell biology, Vol. 151, Issue 1, pp. 187-98, (2000) (PubMed).

Cook, Urrutia, McNiven: "Identification of dynamin 2, an isoform ubiquitously expressed in rat tissues." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 2, pp. 644-8, (1994) (PubMed).

Sontag, Fykse, Ushkaryov, Liu, Robinson, Südhof: "Differential expression and regulation of multiple dynamins." in: The Journal of biological chemistry, Vol. 269, Issue 6, pp. 4547-54, (1994) (PubMed).

Details zu DNM2

Target: DNM2 (Dynamin 2 (DNM2))

Andere Bezeichnung: Dynamin II

Hintergrund: Dynamin is a membrane-associated GTPase that binds to GTP, microtubules, and phospholipids. Northern blot analysis has shown dynamin to be present in most tissues but at an amount about 20-fold lower than that in brain. There are at least two distinct dynamin genes in mammals. Transcripts of both dynamin genes are alternatively spliced at two or more sites. The first site is identical in both dynamins, whereas, the second site differs. The two gene products are known as Dynamin I and Dynamin II and show 79% identity. Dynamin I is expressed almost exclusively in the central nervous system while Dynamin II expression is ubiquitous. The two proteins are highly homologous in the N-terminal region, while the C-terminal domain shows significant divergence. The GTPase activity of Dynamin I is stimulated several fold by binding to microtubules, phospholipids, and membranous vesicles. Dynamin I is a good substrate of PKC in vitro as well as in vivo in resting nerve terminals. In vitro, phosphorylation occurs at the C-terminus of Dynamin I and this enhances the GTPase activity more than 10-fold. However, Dynamin II is not a substrate of PKC and its activity does not appear to be affected by phosphorylation. These data suggest that the function of the common N-terminal domain between Dynamin I and II may be differentially specified by distinct C-terminal domains.

Molekulargewicht: 100 kDa

Pathways: Toll-Like Receptors Cascades