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FLI1 Antikörper

FLI1 Reaktivität: Human WB, IP Wirt: Maus Monoclonal G146-222 unconjugated
Produktnummer ABIN967450
  • Target Alle FLI1 Antikörper anzeigen
    FLI1 (Friend Leukemia Virus Integration 1 (FLI1))
    Reaktivität
    • 73
    • 27
    • 19
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    Human
    Wirt
    • 50
    • 21
    • 3
    Maus
    Klonalität
    • 50
    • 24
    Monoklonal
    Konjugat
    • 53
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser FLI1 Antikörper ist unkonjugiert
    Applikation
    • 62
    • 28
    • 25
    • 17
    • 15
    • 11
    • 7
    • 7
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP)
    Marke
    BD Pharmingen™
    Produktmerkmale
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    Aufreinigung
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Fli-1 ets Domain Fusion Protein
    Klon
    G146-222
    Isotyp
    IgG2b
    Top Product
    Discover our top product FLI1 Primärantikörper
  • Applikationshinweise
    G146-222 has been shown to recognize in vitro translated, recombinant bacterially expressed and endogenous B cell Fli-1. In gel shift assays, this antibody disrupts complexes of Fli-1 and DNA probe, however, this application is not tested.
    Kommentare

    Related Products: ABIN967389

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store undiluted at 4°C.
  • Bailly, Bosselut, Zucman, Cormier, Delattre, Roussel, Thomas, Ghysdael: "DNA-binding and transcriptional activation properties of the EWS-FLI-1 fusion protein resulting from the t(11;22) translocation in Ewing sarcoma." in: Molecular and cellular biology, Vol. 14, Issue 5, pp. 3230-41, (1994) (PubMed).

    May, Lessnick, Braun, Klemsz, Lewis, Lunsford, Hromas, Denny: "The Ewing's sarcoma EWS/FLI-1 fusion gene encodes a more potent transcriptional activator and is a more powerful transforming gene than FLI-1." in: Molecular and cellular biology, Vol. 13, Issue 12, pp. 7393-8, (1994) (PubMed).

  • Target
    FLI1 (Friend Leukemia Virus Integration 1 (FLI1))
    Andere Bezeichnung
    Fli-1 (FLI1 Produkte)
    Synonyme
    EWSR2 antikoerper, SIC-1 antikoerper, FLI1 antikoerper, fli antikoerper, Xfli antikoerper, fli1 antikoerper, Xfli1 antikoerper, fli-1 antikoerper, Xfli-1 antikoerper, MGC121593 antikoerper, Fli-1 antikoerper, Sic1 antikoerper, cb855 antikoerper, wu:fc45b11 antikoerper, xlfli antikoerper, Fli-1 proto-oncogene, ETS transcription factor antikoerper, Fli-1 proto-oncogene, ETS transcription factor S homeolog antikoerper, Friend leukemia integration 1 antikoerper, Fli-1 proto-oncogene, ETS transcription factor a antikoerper, Fli-1 proto-oncogene, ETS transcription factor L homeolog antikoerper, FLI1 antikoerper, Fli1 antikoerper, fli1.S antikoerper, fli1 antikoerper, fli1a antikoerper, fli1.L antikoerper
    Hintergrund
    Fli-1 is a 50 kDa ets-related transcription factor. Like other ets-related proteins it binds to consensus sites in DNA through an 85 amino acid ets domain in the carboxyl region of the protein. Fli-1 is associated with Ewing sarcoma (EWS) through a t(11,22)(q24,q12) chromosomal translocation. This translocation brings the 5' region of the EWS gene into conjunction with the 3' region of the Fli-1 gene encoding the ets-DNA binding domain. Such a translocation is found in 85% of Ewing sarcomas. The resulting fusion protein has the DNA binding activity of Fli- 1 and, with the EWS domain, it is also a more potent transcriptional activator than Fli-1 itself. The strong transforming activity of the EWS-Fli-1 fusion protein may in part be due to its ability to transactivate the promoter for c-myc. G146-222 recognizes human Fli-1. The antibody was raised against a bacterially expressed Fli-1 ets domain fusion protein.
    Molekulargewicht
    50 kDa
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