Biotin Antikörper

Antigen: Biotin

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN95626

Produktbeschreibung

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Format: Lyophilized

Spezifität: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against biotinylated IgG and Biotinylated BSA.

Anwendungen

Applikationshinweise: Recommended Dilutions: This product has been assayed against 1.0 ug of Biotinylated IgG in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code ABIN101990 and (ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:28,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity.

Konzentration: 85.0 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4.

Lagerung: Store vial at 4° C prior to restoration. Restore with 2.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of restoration.

Forschungsgebiet: Signaltransduktion, Metabolismus, Epitop-Tags, Tags/Labels

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Titus JA, Haugland R, Sharrow SO et al.: "Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies." in: J Immunol Methods, Vol. 50, Issue 2, pp. 193-204, 1982 (PubMed).