Biotin Antikörper

Antigen: Biotin

Klonalität: Polyklonal

Wirt: Ziege

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN95611

Produktbeschreibung

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)

Format: Liquid (sterile filtered)

Anwendungen

Applikationshinweise: Suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilution(s): ELISA 1:100,000 - 1:400,000 WESTERN BLOT 1:10,000 - 1:40,000 IMMUNOHISTOCHEMISTRY 1:1,000 - 1:5,000 OTHER APPLICATIONS User Optimized

Konzentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Reinheit: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.

Forschungsgebiet: Signaltransduktion, Metabolismus, Epitop-Tags, Tags/Labels

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).