Vimentin (VIM) Antikörper

Antigen: Vimentin (VIM)

Synonyme: FLJ36605, MGC102095, cb28, MGC139781, VIM, vim, Vim, vime

Klonalität: Monoklonal (VI-RE-1)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Western Blot (WB), ELISA, Immunzytochemie (ICC)

Produktnummer: ABIN94494

Produktbeschreibung

Weitere Bezeichnung: Vimentin

Gen-ID: 10p13

Immunogen: Bacterially expressed full-length human vimentin

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: VI-RE-1

Beschreibung: Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to beexpressed during cell differentiation. All primitive cell types express vimentin but in mostnon-mesenchymal cells it is replaced by other intermediate filament proteins duringdifferentiation. Vimentin is expressed in a wide variety of mesenchymal cell types -fibroblasts, endothelial cells etc., and in a number of other cell types derived frommesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth musclecellsand striated muscle, vimentin is often replaced by desmin, however, duringregeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system(lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts.Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule),endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary andsweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells,vimentin is coexpressed with cytokeratin .Furthermore, vimentin is detected in many cells from the neural crest. Particularlymelanocytes express abundant vimentin. In glial cells vimentin is coexpressed with glialfilament acidic protein (GFAP).Vimentin is present in many different neoplasms but is particulary expressed in thoseoriginated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibroushistiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas,malignant melanoma and schwannoma, are virtually always vimentin positive. Mesodermderived carcinomas like renal cell carcinoma, adrenal cortical carcinoma andadenocarcinomas from endometrium and ovary usually express vimentin. Also thyroidcarcinomas are vimentin positive. Any low differentiated carcinoma may express somevimentin.Vimentin is frequently included in the so-called primary panel (together with CD45,cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpressionof other intermediate filament proteins is strongly suggestive of a mesenchymal tumour ormalignant melanoma.

Spezifität: The antibody VI-RE/1 reacts with human vimentin, a 57 kDa intermediate filament proteinexpressed on a wide variety of mesenchymal and mesodermal cell types. This antibody does not react with: Porcine, Mouse.

Anwendungen

Applikationshinweise: Western Blotting - Recommended dilution: 1-2 µg/ml, overnight in 4 o C Positive control: LEP-19 cell lysate. Negative control: 3T3 mouse cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withreducing Laemmli SDS-PAGE sample buffer. Boil for 3 min in water bath. Application note: Reducing conditions. SDS-PAGE (10% separating gel). Immunocytochemistry - Recommended dilution: Purified Antibody: 5-10 µg/ml. ELISA: The antibody VI-RE/1 recognizes different epitope on human vimentin than the antibodyVI-01 (IgM).

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from hybridoma culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: Abstammungsmarker, Zytoskelett, Neuronale Stammzellmarker, Zell-/Gewebemarker

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Intracellular Flow Cytometry analysis