Immunoglobulin G F(ab)2 Fragment (IgG) Antikörper

Antigen: Immunoglobulin G F(ab)2 Fragment (IgG)

Klonalität: Monoklonal (4A11)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Western Blot (WB), ELISA

Produktnummer: ABIN94400

Produktbeschreibung

Weitere Bezeichnung: IgG (Fab)

Immunogen: Purified human IgG.

Format: Purified

Isotyp: IgG2b

Klon: 4A11

Beschreibung: Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effectormolecule of the humoral immune response in man. Its concentration in blood plasma ofhealthy individuals is approximately 10 g/l, which accounts for about 75% of the total plasmaimmunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days)and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rateas other immunoglobulins.

Spezifität: The antibody 4A11 reacts with Fab fragment of human IgG.

Anwendungen

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from ascites by precipitation methods and ion exchange chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8°C. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. References: *Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975;194:77-95. *Fuller JM, Keyser JW: Serum immunoglobulins after surgical operation. Clin Chem. 1975 May;21(6):667-71. *Balogh Z, Merétey K, Falus A, Bozsóky S: Serological abnormalities in juvenile chronic arthritis: a review of 46 cases. Ann Rheum Dis. 1980 Apr;39(2):129-34. *Brinkmann V, Heusser CH: T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152(2):323-32.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western Blotting