Neural Cell Adhesion Molecule 1 (NCAM1) Antikörper (APC)

Antigen: Neural Cell Adhesion Molecule 1 (NCAM1)

Synonyme: CD56, NCAM, MSK39, ncam, fj56g09, wu:fj56g09, NCAM1, n-cam, LOC397761

Klonalität: Monoklonal (MEM-188)

Wirt: Maus

Reaktivität: Human, Primaten

Konjugat: APC

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN94191

Produktbeschreibung

Weitere Bezeichnung: CD56

Immunogen: KG-1 human acute myelogenous leukemia cell line

Isotyp: IgG2a

Klon: MEM-188

Beschreibung: CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein ofimmunoglobulin family serving as adhesive molecule which is ubiquitously expressed innervous system, usually as 120 kDa, 140 kDa or 180 kDa isoform, and it is also found on Tcells and NK cells. Polysialic modification results in reduction of CD56-mediated celladhesion and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity.CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrinetumours and ovarian granulosa cell tumours.

Spezifität: The antibody MEM-188 reacts with a 180 kDa isoform of CD56 (NCAM) expressed inleukocytes. It has been suggested that the antibody MEM-188 could react with rhesusmonkey lymphocytes. Reactivity with other NCAM isoforms has not been tested.HLDA VI, WS code A055HLDA VI, WS Code NK26HLDA VII, WS code 70077

Anwendungen

Applikationshinweise: The reagent is designed for Flow Cytometry analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Lagerung: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Forschungsgebiet: CD Antigene, Oberflächenrezeptoren der Immunzellen , Signaltransduktion

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Brdicka, Imrich, Angelisovua et al.: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, 2002 (PubMed).

Brdicková, Brdicka, Angelisová et al.: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003 (PubMed).

Lin, Liu, Chen et al.: "CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features." in: Blood, Vol. 106, Issue 10, pp. 3567-74, 2005 (PubMed).

Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Olsen, Tollefsen, Aagaard et al.: "Isolation of Mycobacterium avium subspecies paratuberculosis reactive CD4 T cells from intestinal biopsies of Crohn's disease patients." in: PLoS ONE, Vol. 4, Issue 5, pp. e5641, 2009 (PubMed).

Benson, Grobarova, Richter et al.: "Glycosylation regulates NK cell-mediated effector function through PI3K pathway." in: International immunology, Vol. 22, Issue 3, pp. 167-77, 2010 (PubMed).