CD19 Molecule (CD19) Antikörper (Biotin)

Antigen: CD19 Molecule (CD19)

Synonyme: B4, MGC12802, AW495831, SPNS1, MGC109570, CD19

Klonalität: Monoklonal (LT19)

Wirt: Maus

Reaktivität: Human

Konjugat: Biotin

Produktnummer: ABIN94014

Produktbeschreibung

Weitere Bezeichnung: CD19

Immunogen: Daudi human Burkitt lymphoma cell line

Isotyp: IgG1

Klon: LT19

Beschreibung: CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the timeof heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complexwith CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (Bcell antigen receptor), this complex signals to decrease B cell treshold for activation by theantigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signalindependently of BCR coligation and it turns out to be a central regulatory component uponwhich multiple signaling pathways converge. Mutation of the CD19 gene results inhypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.

Spezifität: The antibody LT19 reacts with CD19 (B4), a 95 kDa type I transmembrane glycoprotein(immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells, it islost on plasma cells.

Anwendungen

Applikationshinweise: Biotinylated antibody is designed for indirect immunofluorescence analysis by FlowCytometry. Suggested working dilution is 1: 200.Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Konzentration: 1 mg/ml

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: Stammzellen, Hämopoetische Vorläufer, Hämatopoetische Stammzellen, Adaptive Immunität, CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Surface staining of human peripheral blood cells with anti-human CD19 (LT19) APC.

Publikationen

Publikationen: Vantourout, Martinez, Fabre et al.: "Ecto-F1-ATPase and MHC-class I close association on cell membranes." in: Molecular immunology, Vol. 45, Issue 2, pp. 485-92, 2007 (PubMed).

Shao, Suresh, Vakil et al.: "Pivotal Advance: Th-1 cytokines inhibit, and Th-2 cytokines promote fibrocyte differentiation." in: Journal of leukocyte biology, Vol. 83, Issue 6, pp. 1323-33, 2008 (PubMed).

Barat, Gilbert, Imbeault et al.: "Extracellular ATP reduces HIV-1 transfer from immature dendritic cells to CD4+ T lymphocytes." in: Retrovirology, Vol. 5, pp. 30, 2008 (PubMed).

Allen, Pang, Skowera et al.: "Plasmacytoid dendritic cells are proportionally expanded at diagnosis of type 1 diabetes and enhance islet autoantigen presentation to T-cells through immune complex capture." in: Diabetes, Vol. 58, Issue 1, pp. 138-45, 2008 (PubMed).

Harrison, Franklin, Campbell: "Enumeration of blood dendritic cells in patients with multiple myeloma at presentation and through therapy." in: Leukemia & lymphoma, Vol. 49, Issue 12, pp. 2272-83, 2008 (PubMed).

Svensson: "Isolation and culture of human hematopoietic progenitors for studies of dendritic cell biology." in: Methods in molecular biology (Clifton, N.J.), Vol. 531, pp. 187-202, 2009 (PubMed).

Gonucalves, Castro, Henriques et al.: "Molecular cloning and analysis of SSc5D, a new member of the scavenger receptor cysteine-rich superfamily." in: Molecular immunology, Vol. 46, Issue 13, pp. 2585-96, 2009 (PubMed).

Rayment, Kooij, Zhang et al.: "Evidence for the specificity for platelet HPA-1a alloepitope and the presenting HLA-DR52a of diverse antigen-specific helper T cell clones from alloimmunized mothers." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 1, pp. 677-86, 2009 (PubMed).