Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) Antikörper

Antigen: Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A)

Synonyme: CD16, FCG3, CD16A, FCGR3, IGFR3, FCR-10, FCRIII, FCGRIII, FCRIIIA

Klonalität: Monoklonal (MEM-168)

Wirt: Maus

Reaktivität: Human, Primaten, Schwein

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN93997

Produktbeschreibung

Weitere Bezeichnung: CD16

Immunogen: Human granulocytes

Format: Purified

Isotyp: IgM

Klon: MEM-168

Beschreibung: CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor.Human FcgammaRIII is expressed in two forms FcgammaRIII-A and -B. FcgammaRIII-A isa transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It isassociated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NKcell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-betasubunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE.FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and isinvolved in their activation and induction of a proadhesive phenotype.

Spezifität: The antibody MEM-168 reacts with CD16 antigen, a low affinity receptor for aggregated IgG(FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA,50-65 kDa, expressed on NK-cells, monocytes and macrophages) and CD16b(FcgammaRIIIB, 48 kDa, mainly expressed on neutrophils).

Anwendungen

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from ascites by gel filtration and precipitation methods.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: Stammzellen, Hämopoetische Vorläufer, Hämatopoetische Stammzellen, CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Flow Cytometry analysis

Publikationen

Publikationen: Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).