Fc Fragment of IgG, Low Affinity IIIb, Receptor (CD16b) (FCGR3B) Antikörper (FITC)

Antigen: Fc Fragment of IgG, Low Affinity IIIb, Receptor (CD16b) (FCGR3B)

Synonyme: CD16, FCG3, CD16b, FCGR3

Klonalität: Monoklonal (MEM-154)

Wirt: Maus

Reaktivität: Human

Konjugat: FITC

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN93995

Produktbeschreibung

Weitere Bezeichnung: CD16b

Immunogen: Human granulocytes

Isotyp: IgG1

Klon: MEM-154

Beschreibung: CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor.Human FcgammaRIII is expressed in two forms FcgammaRIII-A and -B. FcgammaRIII-A isa transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It isassociated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NKcell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-betasubunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE.FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and isinvolved in their activation and induction of a proadhesive phenotype.

Spezifität: The antibody MEM-154 reacts with the epitope on CD16 antigen that residing in proximity toFG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG(FcgammaRIII antigen). The antibody MEM-154 reacts with CD16+ granulocytes.HLDA V, WS Code M MA068HLDA V, WS Code NK NK51

Anwendungen

Applikationshinweise: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Lagerung: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Forschungsgebiet: CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Rose, Foley, Yi et al.: "Cholesterol is obligatory for polarization and chemotaxis but not for endocytosis and associated signaling from chemoattractant receptors in human neutrophils." in: Journal of biomedical science, Vol. 15, Issue 4, pp. 441-61, 2008 (PubMed).

Soehnlein, Kai-Larsen, Frithiof et al.: "Neutrophil primary granule proteins HBP and HNP1-3 boost bacterial phagocytosis by human and murine macrophages." in: The Journal of clinical investigation, Vol. 118, Issue 10, pp. 3491-502, 2008 (PubMed).

Jursik, Prchal, Grillari-Voglauer et al.: "Large-scale production and characterization of novel CD4+ cytotoxic T cells with broad tumor specificity for immunotherapy." in: Molecular cancer research : MCR, Vol. 7, Issue 3, pp. 339-53, 2009 (PubMed).

Schnueriger, Grau, Sondermann et al.: "Development of a quantitative, cell-line based assay to measure ADCC activity mediated by therapeutic antibodies." in: Molecular immunology, Vol. 48, Issue 12-13, pp. 1512-7, 2011 (PubMed).